Biomechanics in health and disease:
advanced physical tools for innovative early diagnosis H2020-MSCA-ITN-2018 n. 812772

Dissemination and Outreach

Table of Contents

Dissemination

Publications

  1. Novel native extracellular matrix probes to target patient- and tissue-specific cell-microenvironment interactions by force spectroscopy. H. Holuigue, L. Nacci, P. Di Chiaro, M. Chighizola, I. Locatelli, C. Schulte, M. Alfano, G.R. Diaferia, A. Podestà bioRxiv 2022.12.02.518867; doi: https://doi.org/10.1101/2022.12.02.518867.
  2. Advances in mechanical biomarkers. Eroles, M. and Rico, F. (2023), J Mol Recognit. https://doi.org/10.1002/jmr.3022
  3. Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells. Falcones, B.; Sanz-Fraile, H.; Marhuenda, E.; Mendizábal, I.; Cabrera-Aguilera, I.; Malandain, N.; Uriarte, J.J.; Almendros, I.; Navajas, D.; Weiss, D.J.; Farré, R.; Otero, J.  Polymers 2021, 13, 2350. https://doi.org/10.3390/polym13142350
  4. Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Carla Silva Martins, Cyntia Taveneau, Gerard Castro-Linares, Mikhail Baibakov, Nicolas Buzhinsky, Mar Eroles, Violeta Milanovi?, Shizue Omi, Jean-Denis Pedelacq, Francois Iv, Léa Bouillard, Alexander Llewellyn, Maxime Gomes, Mayssa Belhabib, Mira Kuzmi?, Pascal Verdier-Pinard, Stacey Lee, Ali Badache, Sanjay Kumar, Cristel Chandre, Sophie Brasselet, Felix Rico, Olivier Rossier, Gijsje H. Koenderink, Jerome Wenger, Stéphanie Cabantous, Manos Mavrakis;  J Cell Biol 6 March 2023; 222 (3): e202203016. doi: https://doi.org/10.1083/jcb.202203016
  5. The nanomechanical fingerprint of colorectal cancer -derived peritoneal metastasis. Ewelina Lorenc, Luca Varinelli, Matteo Chighizola, Silvia Brich, Federica Pisati, Marcello Guaglio, Dario Baratti, Marcello Deraco, Manuela Gariboldi, Alessandro Podesta. bioRxiv 2022.08.17.504271; doi: https://doi.org/10.1101/2022.08.17.504271. 
  6. Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages. Mar Eroles, Javier Lopez-Alonso, Alexandre Ortega, Thomas Boudier, Khaldoun Gharzeddine, Frank Lafont, Clemens M. Franz, Arnaud Millet, Claire Valoteau, Felix Rico. bioRxiv 2022.11.29.518356; doi: https://doi.org/10.1101/2022.11.29.518356.
  7. Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases. Luca Varinelli, Marcello Guaglio, Silvia Brich, Susanna Zanutto, Antonino Belfiore, Federica Zanardi, Fabio Iannelli, Amanda Oldani, Elisa Costa, Matteo Chighizola, Ewelina Lorenc, Simone P Minardi, Stefano Fortuzzi, Martina Filugelli, Giovanna Garzone, Federica Pisati, Manuela Vecchi, Giancarlo Pruneri, Shigeki Kusamura, Dario Baratti, Laura Cattaneo, Dario Parazzoli, Alessandro Podestà, Massimo Milione, Marcello Deraco, Marco A Pierotti, Manuela Gariboldi, Journal of Molecular Cell Biology, 2022;mjac064, https://doi.org/10.1093/jmcb/mjac064.
  8. Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence. Martinez-Vidal, L., Chighizola, M., Berardi, M. et al. Commun Biol 6, 217 (2023). https://doi.org/10.1038/s42003-023-04572-0.
  9. Comparison of Rheological Properties of Healthy versus Dupuytren Fibroblasts When Treated with a Cell Contraction Inhibitor by Atomic Force Microscope. Pérez-Domínguez, S.; López-Alonso, J.; Lafont, F.; Radmacher, M.  Int. J. Mol. Sci. 2023, 24, 2043.https://doi.org/10.3390/ijms24032043.
  10. Rheological comparison between control and Dupuytren fibroblasts when plated in circular micropatterns using atomic force microscopy. Pérez-Domínguez S, Werkmeister E, Marini ML, Dupres V, Janel S, Lafont F and Radmacher M (2022) Front. Phys. 10:1052203. doi: 10.3389/fphy.2022.1052203
  11. Force Sensing on Cells and Tissues by Atomic Force Microscopy. Hatice Holuigue, Ewelina Lorenc, Matteo Chighizola, Carsten Schulte, Luca Varinelli, Marcello Deraco, Marcello Guaglio, Manuela Gariboldi, and Alessandro Podestà. 2022. Sensors 22, no. 6: 2197. https://doi.org/10.3390/s22062197
  12. Novel Decellularization Method for Tissue Slices. Maria Narciso, Anna Ulldemolins, Constança Júnior, Jorge Otero, Daniel Navajas, Ramon Farré, Núria Gavara and Isaac Almendros (2022). Front. Bioeng. Biotechnol. 10:832178. doi: 10.3389/fbioe.2022.832178
  13. Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer. Marie Malier, Magali Court, Khaldoun Gharzeddine, Marie-Hélène Laverierre, Sabrina Marsili, Fabienne Thomas, Thomas Decaens, Gael Roth, Arnaud Millet (2021).Cancer Research doi: 10.1158/0008-5472.CAN-21-1572
  14. Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis. Constança Júnior, Maria Narciso, Esther Marhuenda, Isaac Almendros, Ramon Farré, Daniel Navajas,  Jorge Otero and Núria Gavara. Int. J. Mol. Sci. 2021, 22(23), 12928; https://doi.org/10.3390/ijms222312928
  15. Image-Based Method to Quantify Decellularization of Tissue Sections. Maria Narciso, Jorge Otero, Daniel Navajas, Ramon Farré, Isaac Almendros and Núria Gavara. Int. J. Mol. Sci. 2021, 22(16), 8399; https://doi.org/10.3390/ijms22168399.
  16. Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy, Katerina Karkali, Ignasi Jorba, Daniel Navajas, Enrique Martin-Blanco. STAR Protocols,Volume 3, Issue 4,2022,101901,ISSN 2666-1667: https://doi.org/10.1016/j.xpro.2022.101901.
  17. Causal contributors to tissue stiffness and clinical relevance in urology. Laura Martinez-Vidal, Valentina Murdica, Chiara Venegoni, Filippo Pederzoli, Marco Bandini, Andrea Necchi, Andrea Salonia & Massimo Alfano. Communications Biology volume 4, Article number: 1011 (2021).
  18. Multi-Step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis., Júnior, C.; Ulldemolins, A.; Narciso, M.; Almendros, I.; Farré, R.; Navajas, D.; López, J.; Eroles, M.; Rico, F.; Gavara, N.  Int. J. Mol. Sci. 2023, 24, 1708. https://doi.org/10.3390/ijms24021708.
  19. Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system. Lolo, FN., Walani, N., Seemann, E. et al.  Nat Cell Biol 25, 120–133 (2023).https://doi.org/10.1038/s41556-022-01034-3.
  20. Viscoelastic properties of suspended cells measured with shear flow deformation cytometry. Richard Gerum, Elham Mirzahossein, Mar Eroles, Jennifer Elsterer, Astrid Mainka, Andreas Bauer, Selina Sonntag, Alexander Winterl, Johannes Bartl, Lena Fischer, Shada Abuhattum, Ruchi Goswami, Salvatore Girardo, Jochen Guck, Stefan Schrüfer, Nadine Ströhlein, Mojtaba Nosratlo, Harald Herrmann, Dorothea Schultheis, Felix Rico, Sebastian Johannes Müller, Stephan Gekle, Ben Fabry (2022). eLife 11:e78823. https://doi.org/10.7554/eLife.78823.
  21. A Fast and Efficient Decellularization Method for Tissue Slices. Narciso, M., Ulldemolins, A., Júnior, C., Otero, J., Navajas, D., Farré, R., Gavara, N. and Almendros, I. (2022).  Bio-protocol 12(22): e4550. DOI: 10.21769/BioProtoc.4550.
  22. Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution. Massimiliano Berardi, Kevin Bielawski, Niek Rijnveld, Grzegorz Gruca, Hilde Aardema, Leni van Tol, Gijs Wuite & B. Imran Akca. Communications Biology volume 4, Article number: 610 (2021)
  23. A Universal Model for the Log-Normal Distribution of Elasticity in Polymeric Gels and Its Relevance to Mechanical Signature of Biological Tissues. A. Millet. Biology 18 (2021) 64. DOI: 10.3390/biology10010064.
  24. Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level. M. Chighizola, A. Previdi, T. Dini et al., Nanoscale 12, 14708 (2020). DOI: 10.1039/d0nr01991g. Internal link.
  25. αvβ3 integrin expression increases elasticity in human melanoma cells. L.Lacaria, J.Range, W.Goldmann, F.Rico, JL. Alonso. Biochem Bioph Res Comm (2020). DOI: 10.1016/j.bbrc.2020.02.156. Internal link.
  26. Applicability of atomic force microscopy to determine cancer-related changes in cells. Ma?gorzata Lekka. https://doi.org/10.1098/rsta.2021.0346.
  27. Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer. M. Malier, M. Court, K. Gharzeddine et al., BioRxiv 10.15.341123 (2020). DOI: 10.1101/2020.10.15.341123.
  28. Mutated lamin A modulates stiffness in muscle cells. Maria Chatzifrangkeskou, Delf Kah, Janina R. Lange, Wolfgang H. Goldmann, Antoine Muchir, Biochem Biophys Res Comm 529/3, 861-867 (2020). DOI: 10.1016/j.bbrc.2020.05.102. Internal link.
  29. Atomic force microscopy for cell mechanics and diseases. Sandra Pérez-Domínguez, Shruti G. Kulkarni, Carmela Rianna, Manfred Radmacher. Neuroforum 26, 101-109 (2020). DOI: 10.1515/nf-2020-0001. Internal link.
  30. Large colloidal probes for atomic force microscopy: fabrication and calibration issues. M. Chighizola, L. Puricelli, L. Bellon, A. Podestà. J. Molecular recognition (2020). DOI: 10.1002/jmr.2879. Internal link.
  31. Mechanotransduction in neuronal cell development and functioning. M. Chighizola, T. Dini, C. Lenardi et al. Biophys Rev 11, 701–720 (2019). DOI: 10.1007/s12551-019-00587-2. Internal link.
  32. Book : Mechanics of cells and tissues in diseases. Vol. 1: Biomedical Methods. Vol. 2: Biomedical Applications. M. Lekka, D. Navajas, M. Radmacher, A. Podestà. De Gruyter

Posters

  1. Poster. Phys2BioMed - Biomechanics in health and disease: advanced physical tools for innovative early diagnosis. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Podestà, Alessandro (1); Pellequer, Jean-Luc (2); Lafont, Frank (3); Millet, Arnaud (4); Navajs, Daniel (5); Farré, Ramon (5); Rico, Felix (6); Schillers, Hermann (7); Lekka, Malgorzata (8); Alfano, Massimo (9); Radmacher, Manfred (10); Bielawski, Kevin (11); Rijnveld, Niek (11); Iannuzzi, Davide (11); Laidler, Piotr (12); Copin, Marie-Christine (13); Goldmann, Wolfgang (14); Walter, Benjamin (15); Milani, Pascale (16); Chlasta, Julien (16). Organization(s): 1: UMIL; 2: CEA; 3: CNRS; 4: CHUGR; 5: IBEC; 6: INSERM; 7: WWU; 8: IFJPAN; 9: OSR; 10: UB; 11: O11; 12: JUMC; 13: TMTC-C2RC; 14: FAU; 15: VMICRO; 16: BIOMECA.
  2. Poster ESR1. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Hatice Holuigue. UMIL.
  3. Poster ESR2. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Ewelina Lorenc. UMIL.
  4. Poster ESR4. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Javier Lopez Alonso. CNRS.
  5. Poster ESR5. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Maria Luisa Marini. CNRS.
  6. Poster ESR7. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Constança Júnior. IBEC.
  7. Poster ESR8. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Maria Leonor Narciso. IBEC.
  8. Poster ESR9. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Mar Eroles Navarro. INSERM.
  9. Poster ESR11. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Kajangi Gnanachandran. IFJPAN.
  10. Poster ESR13. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Sandra Pérez Domínguez. UB.
  11. Poster ESR14. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Shruti Kulkarni. UB.
  12. Poster ESR15. 9th AFM BioMed Conference 2019, Munster, 2-6 September 2019. Massimiliano Berardi. O11.
  13. ESR1 and ESR2 at the Nanoengineering for Mechanobiology Conference, 27-29 March 2022, Camogli, Genova, Italy.
  14. ESR7 and ESR8 co-author - American thoracic society 2022 Conference, May 2022, San Francisco, USA (virtual).
  15. ESR8 and co-author in two other posters - American thoracic society 2022 Conference, May 2022, San Francisco, USA (virtual).
  16. ESR8 - ERS (European Respiratory Society) Research Seminar: "Innovative 3D models for understanding mechanisms underlying lung diseases: powerful tools for translational research", 7–8 April 2022, Lisbon, Portugal.
  17. ESR8 - The extracellullar matrix pharmacology congress: ECM 2022 Conference, 23-25 June 2022, Tivoli Congress Center, Copenhagen.
  18. ESR8, two poster - ERS (European Respiratory Society) International Congress 2022, 4-6th September 2022, Barcelona, Spain.
  19. ESR8, e-poster - 14as Jornadas de formanciòn ciberes, October 2021, Barcelona, Spain.
  20. ESR8 - IN2UB 2021 Meeting, October 2012, Barcelona, Spain.
  21. ESR8, e-poster - American thoracic society 2021 Conference, May 2021, San Francisco, USA (virtual).
  22. ESR9, e-poster - Immunobiophysics, EMBO 2021.
  23. ESR9 - EBSA conference 2021, Vienna, Austria.
  24. ESR9 - Biophysical Society Annual Meeting 2021, San Francisco, USA.
  25. ESR11 - 12th symposium "Physics of Cancer", 30 August - 1 September 2021, Leipzig, Germany.  – awarded for the best poster (first place)
  26. ESR11 - NanoScientific Forum Europe - 15-17 September 2021, Freiburg, Germany. – poster awarded as second best poster.
  27. ESR11 - 13th European Biophysics Conference, EBSA 2021
  28. ESR11 - XI Workshop on Applications of Scanning Probe Microscopy – STM/AFM 2022, 30th November - 4th of December 2022, Zakopane, Poland. 
  29. ESR13 - DPG Spring Meeting, BP-CPP-DY-SOE 2021, 22-24 March 2022 (virtual).
  30. ESR13 - MechanochemBio2021- 23-25 August 2021 (virtual)
  31. ESR13 - DGfB2022 25-28 September 2022.

Scientific talks

  1. Analysis of Mechanical Data on Cells. Talk at AFMBioMed 2019 conference in Munster, September 2019. Presenting Author: Radmacher, Manfred.
  2. Studying the cell-microenvironment interaction by adhesive force spectroscopy with colloidal nanostructured probes. Chighizola, Matteo; Schulte, Carsten; Dini, Tania; Previdi, Anita; Piazzoni, Claudio; Lenardi, Cristina; Milani, Paolo; Podestà, Alessandro. Talk at AFMBioMed 2019 conference in Munster, September 2019.  Presenting Author: Podestà, Alessandro.
  3. Changes of biomechanical properties of prostate cancer cells after microtubule targeting therapy – in space and time. Kubiak, Andrzej; Chighizola, Matteo; Bobrowska, Justyna; Suchy, Klaudia; Schulte, Carsten; Podesta, Alessandro; Laidler, Piotr; Lekka, Malgorzata. Talk at  AFMBioMed 2019 conference in Munster, September 2019. Presenting Author: Kubiak, Andrzej.
  4. Fast phenotyping of cell mechanics: Oral presentation at GDR Mecabio in Marseille. December 2019. Mar Eroles-Navarro.
  5. Comparison of viscoelastic properties of NIH-3T3 cells using different tip geometries: Poster Presentation at the Meeting of North-german Biophycists in Borstel. Jan 2020. Shruti Kulkarni, Sandra Pérez-Domínguez, Manfred Radmacher.
  6. Measuring viscoelasticity of cells by atomic force microscopy. Talk at  Arbre Mabieu Plenary Meeting in Prague, March 2020. Presenting Author: Radmacher, Manfred.
  7. Talk at AFMBioMed 2022 conference in Nagoya, Japan
    1. ESR1: "Native ECM-based AFM probes for adhesion force spectroscopy studies of the cell-microenvironment interaction"
    2. ESR2: "Correlation between ECM stiffness distribution and peritoneal metastasis progression"
    3. ESR3: "Nanomechanics on living plant root tissues: Synergistic effect of Iron and Aluminum"
    4. ESR4: "The mechanics of squeezing: automated AFM measurements allows tracking mechanical changes in 2D cell migration"
    5. ESR7: "Mechanical Biomarkers of Pulmonary Fibrosis Assessed by AFM"
    6. ESR9 : "Mechanical phenotyping of monocytes under the cytokine storm"
    7. ESR11 : "Mechanical and microrheological properties of bladder cancer spheroids by Atomic and Hydraulic Force Spectroscopy"
    8. ESR12: "Micro-mechanical fingerprints of bladder in health and disease"
    9. ESR13: "AFM approach to study Dupuytren disease at 2D and 3D level"
    10. ESR14: "Viscoelastic and adhesive properties of pancreatic cancer cells"
    11. ESR16: "Quantification of cellular mechano-response on the single cell level"

Lectures

  1. AFM BioMed Summer School. Grenoble, France. October 2019.
    1. A.Podesta. Introduction to AFM.
    2. L.Redondo. Imaging membrane at high speed.
    3. J.L.Pellequer. AFM imaging in integrated structural biology.
    4. D.Navajas. Micromehcanics of the cell. 
    5. A.Millet. Biology at the tissue level: challenges and opportunities
    6. F.Lafont. Correlative microscopy.
    7. M.Lekka. Nanomechanics characterization of cells and tissues.
    8. M.Radmacher. Cell mechanics.
    9. F.Rico. Single molecule force spectroscopy.
  2. School on Nanomechanics. Bremen, Germany, and online. March 2020.
    1. O11. Presentation of Chiaro nanoindenter. 
    2. F.Rico. Contact mechanics.
    3. D.Navajas. Viscoelasticity.
    4. M.Radmacher. Analysing thermal data.
    5. M.Radmacher. Analysing force curves.

Outreach

Communications to the broad public

  1. European Researchers' Night 2019 : Participation of the ESRs in Krakow, Marseille and Milan. 

  2. Fête de la Science: Participation of the ESRs in Marseille and Lille.

Press 

  1. Atomic Force Microscopy for Cell Mechanics and Diseases. Sandra Pérez-Domínguez, Shruti G Kulkarni, Carmela Rianna and Manfred Radmacher. Neuroforum 2020.
  2. Interview with Prof. M. Lekka for her 30 years of AFM. Women in STEM series, Nunano Interviews, June 2023.

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MOOC

 You can find here the link to the Massive Open Online Course of Phys2BioMed.