ESR13

Project Title

Creep Response of Cells in Diseases

Start date

1st June 2019

Beneficiary

UB

Objectives

Developing instrumentation to measure reliably viscoelastic properties of cells and tissues to determine the mechanical phenotype.

Expected results

This ESR will develop variants of AFM allowing to measure the creep response of cells and tissues. Beyond instrumentation, this also
requires the development of models for the viscoelastic behaviour of cells and tissues and data analysis tools to obtain values for storage and loss moduli. The methods will be tested on cancer cell lines available at UB and IFJPAN, which have been shown to exhibit a well-defined mechanical fingerprint. The elaborated methodology of creep measurements and theoretical models will be applied to evaluate viscoelastic phenotype in cancer tissues. This ESR is shared with IFJPAN in a pilot joint doctorate project.

Planned secondments

1– INSERM, F. Rico (3 months at month 6). Developing models for viscoelastic response of cells & tissues. 2 - IFJPAN, M. Lekka (2 months at month 10). Creep response of cancer cells. 3 - BioMeca, P. Milani (1.5 months at month 13). Validation of analysis of cell mechanics data. 4 - OSR, M. Alfano (1 month at month 18). Preparation of cells and tissues for creep response studies. 5 - IBEC, D. Navajas (2 months at month 24). Creep response of lung cells. 6 – UMIL, A. Podestà (2 months at month 13). Characterisation and Interferometric calibration of AFM probes.
 
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