Biomechanics in health and disease:
advanced physical tools for innovative early diagnosis
H2020-MSCA-ITN-2018 n. 812772

ESR6. Khaldoun Gharzeddine

Short biographic note

Khaldoun I am Khaldoun Gharzeddine, Lebanese born in 1997.

I obtained my Bachelor Degree in Biochemitry from the Lebanese University in 2017. To satistfy my urge to learn and enhance my knowledge and expertise, I enrolled in a Master II program in Immunology, Microbiology and Infectious Diseases at Universite Grenoble Alpes – France, where I graduated in summer 2019. During my Master II, I studied the role of macrophages in the development and progression of Hepatocellular Carcinoma (HCC) in Diethylnitrosmaine (DEN) induced rat model.

Currently, I am working with enthusiasm and motivation on the Phys2BioMed Project as the ESR6 in University Hospital Grenoble Alpes where I will study the nano-mechanical signature of cancerous tissues and its relation with the associated immune response focusing on macrophages.

 

Useful links

E-mail: khaldoun_1997@hotmail.com

 

Project Title

Nano-mechanical signature of cancerous tissues and its relation to the associated immune response

Start date

1st June 2019

Beneficiary

CHUGR

Objectives

Define the use of AFM in diagnostic procedure in human tissues.

Expected results

The ESR will participate to the development of instrumentation, sample preparation and analysis tools for measuring nanomechanics parameters of animal and human tissues obtained directly from the surgery room. AFM analysis will be compared to histological and other structural methods like dynamic light scattering. The ESR will also be involved in the design of soft substrate cell culture (hydrogels) mimicking the ECM to validate in vitro the determination of mechanical properties of these model tissues. One goal of the project is to include AFM data in a multidimensional analysis of tissues to gain information for diagnosis, we will particularly concentrate our attention toward cancerous transformation of fibrotic lesions (cirrhotic) in liver diseases.The ESR will be also involved in scientific communication, training, and dissemination.

Planned secondments

1. CEA-IBS,J.-L. Pellequer (2 months,month 8). Indentation measurement on isolated cells 2. WWU, H. Schillers (3 months,month 14). Force spectroscopy for cell-cell interaction 3. IFJPAN, M.Lekka (2 months,month 18). AFM of cancerous cells 4. INSERM, F. Rico (3 months,month 22). High-speed AFM measurements.