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Table of Contents
The Phys2BioMed MOOC
A Massive Open Online Course on:
Standardization of mechanical measurements on cells and tissues
The Consortium organized the MOOC around the common theme of the Action: the standardization of nanomechanical measurements of cells and tissues.
This is the list of topics included in the MOOC with indication of the lecturers:
INTRODUCTION
1) What is standardization (Jean-Luc Pellequer & Hermann Schillers) (video and PDF)
METHODS
2) Instrument calibrations (Alessandro Podestà)
3) Contact models (Felix Rico)
(video and PDF)
4) Viscoelasticity (Daniel Navajas or Manfred Radmacher)
5) Sample preparation (Frank Lafont and Massimo Alfano)
6) Data processing (Nuria Gavara)
7) Data statistics (Manfred Radmacher)
APPLICATIONS
8) Standardization of nanomechanics on cells (Malgorzata Lekka)
Additional documents
The following documents contain lectures and notes from previous training events of Phys2BioMed and other initiatives involving the Consortium members.
Lectures from Phys2BioMed schools (AFMBioMed Summer School 2019 in Grenoble and Lille 2021)
1) Basic principles of AFM (A. Podestà) (PDF)
2) Single molecule mechanics (F. Rico) (PDF)
3) Measuring mechanical properties of cells and tissues (A. Podestà) (PDF)
4) Cell and ECM microrheology (D. Navajas) (PDF)
5) Mechanics in disease (M. Radmacher) (PDF)
6) Hi-speed AFM (L. Redondo-Morta) (PDF)
7a) Correlative microscopy for mechanobiology (F. Lafont) (PDF)
7b) Correlative microscopy (S. Janel) (video)
8) AFM and structural biology (single molecule) (J-L. Pellequer) (PDF)
9) Biology at the tissue level (A. Millet) (PDF)
Tutorials from AFMBioMed Summer School 2020 (AFM for life sciences and nanomedicine):
videos on Zenodo
AFM recipes from previous COST Action TD1002 (AFM4NanoMed&Bio)
1) Custom adhesive-free colloidal probes (A. Podestà) (PDF)
2) Ozone cleaner DIY (Y. Delcuze and J.-L. Pellequer) (PDF)