phys2biomed_mooc
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| - | ====== The Phys2BioMed MOOC ====== | ||
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| - | A Massive Open Online Course on: | ||
| ====== Standardization of mechanical measurements on cells and tissues ====== | ====== Standardization of mechanical measurements on cells and tissues ====== | ||
| + | |||
| + | **A Massive Open Online Course (MOOC) from the Phys2BioMed project ([[https:// | ||
| The Consortium organized the MOOC around the common theme of the Action: the standardization of nanomechanical measurements of cells and tissues.\\ | The Consortium organized the MOOC around the common theme of the Action: the standardization of nanomechanical measurements of cells and tissues.\\ | ||
| - | This is the list of topics included in the MOOC with indication of the lecturers: | ||
| - | **INTRODUCTION**\\ | + | ===== INTRODUCTION |
| - | 1) What is standardization (Jean-Luc Pellequer & Hermann Schillers) ([[https:// | + | 1) What is standardization (Jean-Luc Pellequer & Hermann Schillers) ([[https:// |
| - | **METHODS**\\ | + | ===== METHODS |
| 2) Instrument calibrations (Alessandro Podestà)\\ | 2) Instrument calibrations (Alessandro Podestà)\\ | ||
| 3) Contact models (Felix Rico) | 3) Contact models (Felix Rico) | ||
| ([[https:// | ([[https:// | ||
| - | 4) Viscoelasticity (Daniel Navajas | + | 4) Viscoelasticity (Daniel Navajas) ([[|video]] and [[https:// |
| 5) Sample preparation (Frank Lafont and Massimo Alfano)\\ | 5) Sample preparation (Frank Lafont and Massimo Alfano)\\ | ||
| 6) Data processing (Nuria Gavara)\\ | 6) Data processing (Nuria Gavara)\\ | ||
| 7) Data statistics (Manfred Radmacher)\\ | 7) Data statistics (Manfred Radmacher)\\ | ||
| - | **APPLICATIONS**\\ | + | ===== APPLICATIONS |
| 8) Standardization of nanomechanics on cells (Malgorzata Lekka)\\ | 8) Standardization of nanomechanics on cells (Malgorzata Lekka)\\ | ||
| + | \\ | ||
| ===== Additional documents ===== | ===== Additional documents ===== | ||
| The following documents contain lectures and notes from previous training events of Phys2BioMed and other initiatives involving the Consortium members.\\ | The following documents contain lectures and notes from previous training events of Phys2BioMed and other initiatives involving the Consortium members.\\ | ||
| - | **Lectures** from Phys2BioMed schools (AFMBioMed Summer School 2019 in Grenoble and Lille 2021) \\ | + | ==== Lectures from Phys2BioMed schools (AFMBioMed Summer School 2019 in Grenoble and Lille 2021) ==== |
| 1) Basic principles of AFM (A. Podestà) ([[https:// | 1) Basic principles of AFM (A. Podestà) ([[https:// | ||
| 2) Single molecule mechanics (F. Rico) ([[https:// | 2) Single molecule mechanics (F. Rico) ([[https:// | ||
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| 8) AFM and structural biology (single molecule) (J-L. Pellequer) ([[https:// | 8) AFM and structural biology (single molecule) (J-L. Pellequer) ([[https:// | ||
| 9) Biology at the tissue level (A. Millet) ([[https:// | 9) Biology at the tissue level (A. Millet) ([[https:// | ||
| + | 10) Cell and tissue mechanics (M. Lekka) ([[https:// | ||
| + | 11) Super resolution microscopy (E. Werkmeister) | ||
| - | **Tutorials** from AFMBioMed Summer School 2020 (AFM for life sciences and nanomedicine): \\ | + | |
| + | |||
| + | ==== Tutorials from AFMBioMed Summer School 2020 (AFM for life sciences and nanomedicine) | ||
| [[https:// | [[https:// | ||
| - | **AFM recipes** from previous COST Action TD1002 (AFM4NanoMed& | + | Topics:\\ |
| + | 1) AFM Surface Coating (S. Fidan, C. Valotteau, F. Rico) \\ | ||
| + | 2) Tutorial for High-Speed AFM (HS-AFM) imaging (I. Casuso) \\ | ||
| + | 3) Mica sample substrate preparation for AFM (N. Buzhinsky) \\ | ||
| + | 4) Cell mechanics measurements in force mapping (L. Lacaria, F. Rico) \\ | ||
| + | 5) AFM calibration (F. Rico, J. Rodriguez-Ramos) \\ | ||
| + | |||
| + | ==== AFM recipes from previous COST Action TD1002 (AFM4NanoMed& | ||
| 1) Hydrophobic substrates for adhesion of biocolloids (M. Papi) ([[https:// | 1) Hydrophobic substrates for adhesion of biocolloids (M. Papi) ([[https:// | ||
| 2) Custom adhesive-free colloidal probes (A. Podestà) ([[https:// | 2) Custom adhesive-free colloidal probes (A. Podestà) ([[https:// | ||
| 3) Ozone cleaner DIY (Y. Delcuze and J.-L. Pellequer) ([[https:// | 3) Ozone cleaner DIY (Y. Delcuze and J.-L. Pellequer) ([[https:// | ||
| - | 4) Mild fixation of cells for AFM experiments (H. Oberleithner)([[https:// | + | 4) Mild fixation of cells for AFM experiments (H. Oberleithner) ([[https:// |
| 5) Cleaning silicon and gold-coated substrates for SPM measurements (R. Panajotović) ([[https:// | 5) Cleaning silicon and gold-coated substrates for SPM measurements (R. Panajotović) ([[https:// | ||
| - | 6) Creating an XY calibration sample for AFM using a DVD (B. Tiribilli) | + | 6) Creating an XY calibration sample for AFM using a DVD (B. Tiribilli) |
| - | ([[([[https:// | + | ([[https:// |
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